unable to find an inherited method for function 'rowData' for signature '“logical”'





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I have an R markdown code chunk that looks like this ( it is a small part of an extremely large code).



  filterLowExpressedGenes <- function(sce, cutoffCell, cutoffTranscript){

apply(
counts(sce[ , colData(sce)$use]),
1,
function(x) length(x[x > cutoffTranscript]) >= cutoffCell)
}
```


```{r include=T}
for (i in seq_along(sce_list))
{
print(rowData(sce_list[[i]])$use <- filterLowExpressedGenes(sce_list[[i]], 3, 5))
}

```


{r include=T}
for (i in seq_along(sce_list))
{
print(table(rowData(sce_list[[i]])$use))
}



Till here all works fine and the output is:



## [1] "sce_1"
##
## FALSE TRUE
## 31852 1842
## [1] "sce_2"
##
## FALSE TRUE
## 31009 2685
## [1] "sce_3"
##
## FALSE TRUE
## 31560 2134
## [1] "sce_4"
##
## FALSE TRUE
## 31697 1997


The next chunk that operates on the information processed in the previous chunks throws up error:



code: 
```{r include=T}
for (i in seq_along(sce_list))
{
sce_list[[i]]$qc = sce_list[[i]][rowData(sce_list[[i]]$use), colData(sce_list[[i]]$use)]
}

```


The error that I get is:



    Quitting from lines 331-345 (prototype.Rmd) 
Error in (function (classes, fdef, mtable) :
unable to find an inherited method for function 'rowData' for signature '"logical"'
Calls: <Anonymous> ... withVisible -> eval -> eval -> [ -> rowData -> <Anonymous>
Execution halted


I am sorry, I cannot provide you the sample workable data. As this is somewhat in between coding portion and that means that data has already gone under some sort of processing.



I am fully aware that it will require some guess work or hit and trial to figure out the solution. But any help/suggestion to make this work is welcome.



Thank you in advance.










share|improve this question

























  • You are placing the $usein the wrong place, I believe it should be rowData(sce_list[[i]]$use), it is a member of the list, not from the function's return value.

    – Rui Barradas
    Nov 25 '18 at 21:26






  • 2





    use cat() to print out the value of i as you go along, you can see where the error occurs. Then inspect the relevant element of sce_list ...

    – Ben Bolker
    Nov 25 '18 at 22:29











  • @RuiBarradas: Hi, Thank you for your comment. I did move little forward. what I did was sce_list[[i]]$qc = sce_list[[i]][rowData(sce_list[[i]]$use), colData(sce_list[[i]]$use)] Now I have a new error message Error in (function (classes, fdef, mtable) : unable to find an inherited method for function 'rowData' for signature '"logical"' Calls: <Anonymous> ... withVisible -> eval -> eval -> [ -> rowData -> <Anonymous> Execution halted

    – Angelo
    Nov 25 '18 at 22:57











  • What package contains rowData?

    – user2554330
    Nov 27 '18 at 0:27











  • Hi, The package scater used for single cell experiment data analysis.

    – Angelo
    Nov 27 '18 at 8:45


















0















I have an R markdown code chunk that looks like this ( it is a small part of an extremely large code).



  filterLowExpressedGenes <- function(sce, cutoffCell, cutoffTranscript){

apply(
counts(sce[ , colData(sce)$use]),
1,
function(x) length(x[x > cutoffTranscript]) >= cutoffCell)
}
```


```{r include=T}
for (i in seq_along(sce_list))
{
print(rowData(sce_list[[i]])$use <- filterLowExpressedGenes(sce_list[[i]], 3, 5))
}

```


{r include=T}
for (i in seq_along(sce_list))
{
print(table(rowData(sce_list[[i]])$use))
}



Till here all works fine and the output is:



## [1] "sce_1"
##
## FALSE TRUE
## 31852 1842
## [1] "sce_2"
##
## FALSE TRUE
## 31009 2685
## [1] "sce_3"
##
## FALSE TRUE
## 31560 2134
## [1] "sce_4"
##
## FALSE TRUE
## 31697 1997


The next chunk that operates on the information processed in the previous chunks throws up error:



code: 
```{r include=T}
for (i in seq_along(sce_list))
{
sce_list[[i]]$qc = sce_list[[i]][rowData(sce_list[[i]]$use), colData(sce_list[[i]]$use)]
}

```


The error that I get is:



    Quitting from lines 331-345 (prototype.Rmd) 
Error in (function (classes, fdef, mtable) :
unable to find an inherited method for function 'rowData' for signature '"logical"'
Calls: <Anonymous> ... withVisible -> eval -> eval -> [ -> rowData -> <Anonymous>
Execution halted


I am sorry, I cannot provide you the sample workable data. As this is somewhat in between coding portion and that means that data has already gone under some sort of processing.



I am fully aware that it will require some guess work or hit and trial to figure out the solution. But any help/suggestion to make this work is welcome.



Thank you in advance.










share|improve this question

























  • You are placing the $usein the wrong place, I believe it should be rowData(sce_list[[i]]$use), it is a member of the list, not from the function's return value.

    – Rui Barradas
    Nov 25 '18 at 21:26






  • 2





    use cat() to print out the value of i as you go along, you can see where the error occurs. Then inspect the relevant element of sce_list ...

    – Ben Bolker
    Nov 25 '18 at 22:29











  • @RuiBarradas: Hi, Thank you for your comment. I did move little forward. what I did was sce_list[[i]]$qc = sce_list[[i]][rowData(sce_list[[i]]$use), colData(sce_list[[i]]$use)] Now I have a new error message Error in (function (classes, fdef, mtable) : unable to find an inherited method for function 'rowData' for signature '"logical"' Calls: <Anonymous> ... withVisible -> eval -> eval -> [ -> rowData -> <Anonymous> Execution halted

    – Angelo
    Nov 25 '18 at 22:57











  • What package contains rowData?

    – user2554330
    Nov 27 '18 at 0:27











  • Hi, The package scater used for single cell experiment data analysis.

    – Angelo
    Nov 27 '18 at 8:45














0












0








0








I have an R markdown code chunk that looks like this ( it is a small part of an extremely large code).



  filterLowExpressedGenes <- function(sce, cutoffCell, cutoffTranscript){

apply(
counts(sce[ , colData(sce)$use]),
1,
function(x) length(x[x > cutoffTranscript]) >= cutoffCell)
}
```


```{r include=T}
for (i in seq_along(sce_list))
{
print(rowData(sce_list[[i]])$use <- filterLowExpressedGenes(sce_list[[i]], 3, 5))
}

```


{r include=T}
for (i in seq_along(sce_list))
{
print(table(rowData(sce_list[[i]])$use))
}



Till here all works fine and the output is:



## [1] "sce_1"
##
## FALSE TRUE
## 31852 1842
## [1] "sce_2"
##
## FALSE TRUE
## 31009 2685
## [1] "sce_3"
##
## FALSE TRUE
## 31560 2134
## [1] "sce_4"
##
## FALSE TRUE
## 31697 1997


The next chunk that operates on the information processed in the previous chunks throws up error:



code: 
```{r include=T}
for (i in seq_along(sce_list))
{
sce_list[[i]]$qc = sce_list[[i]][rowData(sce_list[[i]]$use), colData(sce_list[[i]]$use)]
}

```


The error that I get is:



    Quitting from lines 331-345 (prototype.Rmd) 
Error in (function (classes, fdef, mtable) :
unable to find an inherited method for function 'rowData' for signature '"logical"'
Calls: <Anonymous> ... withVisible -> eval -> eval -> [ -> rowData -> <Anonymous>
Execution halted


I am sorry, I cannot provide you the sample workable data. As this is somewhat in between coding portion and that means that data has already gone under some sort of processing.



I am fully aware that it will require some guess work or hit and trial to figure out the solution. But any help/suggestion to make this work is welcome.



Thank you in advance.










share|improve this question
















I have an R markdown code chunk that looks like this ( it is a small part of an extremely large code).



  filterLowExpressedGenes <- function(sce, cutoffCell, cutoffTranscript){

apply(
counts(sce[ , colData(sce)$use]),
1,
function(x) length(x[x > cutoffTranscript]) >= cutoffCell)
}
```


```{r include=T}
for (i in seq_along(sce_list))
{
print(rowData(sce_list[[i]])$use <- filterLowExpressedGenes(sce_list[[i]], 3, 5))
}

```


{r include=T}
for (i in seq_along(sce_list))
{
print(table(rowData(sce_list[[i]])$use))
}



Till here all works fine and the output is:



## [1] "sce_1"
##
## FALSE TRUE
## 31852 1842
## [1] "sce_2"
##
## FALSE TRUE
## 31009 2685
## [1] "sce_3"
##
## FALSE TRUE
## 31560 2134
## [1] "sce_4"
##
## FALSE TRUE
## 31697 1997


The next chunk that operates on the information processed in the previous chunks throws up error:



code: 
```{r include=T}
for (i in seq_along(sce_list))
{
sce_list[[i]]$qc = sce_list[[i]][rowData(sce_list[[i]]$use), colData(sce_list[[i]]$use)]
}

```


The error that I get is:



    Quitting from lines 331-345 (prototype.Rmd) 
Error in (function (classes, fdef, mtable) :
unable to find an inherited method for function 'rowData' for signature '"logical"'
Calls: <Anonymous> ... withVisible -> eval -> eval -> [ -> rowData -> <Anonymous>
Execution halted


I am sorry, I cannot provide you the sample workable data. As this is somewhat in between coding portion and that means that data has already gone under some sort of processing.



I am fully aware that it will require some guess work or hit and trial to figure out the solution. But any help/suggestion to make this work is welcome.



Thank you in advance.







r knitr






share|improve this question















share|improve this question













share|improve this question




share|improve this question








edited Nov 26 '18 at 9:58







Angelo

















asked Nov 24 '18 at 9:29









AngeloAngelo

1,70462345




1,70462345













  • You are placing the $usein the wrong place, I believe it should be rowData(sce_list[[i]]$use), it is a member of the list, not from the function's return value.

    – Rui Barradas
    Nov 25 '18 at 21:26






  • 2





    use cat() to print out the value of i as you go along, you can see where the error occurs. Then inspect the relevant element of sce_list ...

    – Ben Bolker
    Nov 25 '18 at 22:29











  • @RuiBarradas: Hi, Thank you for your comment. I did move little forward. what I did was sce_list[[i]]$qc = sce_list[[i]][rowData(sce_list[[i]]$use), colData(sce_list[[i]]$use)] Now I have a new error message Error in (function (classes, fdef, mtable) : unable to find an inherited method for function 'rowData' for signature '"logical"' Calls: <Anonymous> ... withVisible -> eval -> eval -> [ -> rowData -> <Anonymous> Execution halted

    – Angelo
    Nov 25 '18 at 22:57











  • What package contains rowData?

    – user2554330
    Nov 27 '18 at 0:27











  • Hi, The package scater used for single cell experiment data analysis.

    – Angelo
    Nov 27 '18 at 8:45



















  • You are placing the $usein the wrong place, I believe it should be rowData(sce_list[[i]]$use), it is a member of the list, not from the function's return value.

    – Rui Barradas
    Nov 25 '18 at 21:26






  • 2





    use cat() to print out the value of i as you go along, you can see where the error occurs. Then inspect the relevant element of sce_list ...

    – Ben Bolker
    Nov 25 '18 at 22:29











  • @RuiBarradas: Hi, Thank you for your comment. I did move little forward. what I did was sce_list[[i]]$qc = sce_list[[i]][rowData(sce_list[[i]]$use), colData(sce_list[[i]]$use)] Now I have a new error message Error in (function (classes, fdef, mtable) : unable to find an inherited method for function 'rowData' for signature '"logical"' Calls: <Anonymous> ... withVisible -> eval -> eval -> [ -> rowData -> <Anonymous> Execution halted

    – Angelo
    Nov 25 '18 at 22:57











  • What package contains rowData?

    – user2554330
    Nov 27 '18 at 0:27











  • Hi, The package scater used for single cell experiment data analysis.

    – Angelo
    Nov 27 '18 at 8:45

















You are placing the $usein the wrong place, I believe it should be rowData(sce_list[[i]]$use), it is a member of the list, not from the function's return value.

– Rui Barradas
Nov 25 '18 at 21:26





You are placing the $usein the wrong place, I believe it should be rowData(sce_list[[i]]$use), it is a member of the list, not from the function's return value.

– Rui Barradas
Nov 25 '18 at 21:26




2




2





use cat() to print out the value of i as you go along, you can see where the error occurs. Then inspect the relevant element of sce_list ...

– Ben Bolker
Nov 25 '18 at 22:29





use cat() to print out the value of i as you go along, you can see where the error occurs. Then inspect the relevant element of sce_list ...

– Ben Bolker
Nov 25 '18 at 22:29













@RuiBarradas: Hi, Thank you for your comment. I did move little forward. what I did was sce_list[[i]]$qc = sce_list[[i]][rowData(sce_list[[i]]$use), colData(sce_list[[i]]$use)] Now I have a new error message Error in (function (classes, fdef, mtable) : unable to find an inherited method for function 'rowData' for signature '"logical"' Calls: <Anonymous> ... withVisible -> eval -> eval -> [ -> rowData -> <Anonymous> Execution halted

– Angelo
Nov 25 '18 at 22:57





@RuiBarradas: Hi, Thank you for your comment. I did move little forward. what I did was sce_list[[i]]$qc = sce_list[[i]][rowData(sce_list[[i]]$use), colData(sce_list[[i]]$use)] Now I have a new error message Error in (function (classes, fdef, mtable) : unable to find an inherited method for function 'rowData' for signature '"logical"' Calls: <Anonymous> ... withVisible -> eval -> eval -> [ -> rowData -> <Anonymous> Execution halted

– Angelo
Nov 25 '18 at 22:57













What package contains rowData?

– user2554330
Nov 27 '18 at 0:27





What package contains rowData?

– user2554330
Nov 27 '18 at 0:27













Hi, The package scater used for single cell experiment data analysis.

– Angelo
Nov 27 '18 at 8:45





Hi, The package scater used for single cell experiment data analysis.

– Angelo
Nov 27 '18 at 8:45












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